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Research article2006Peer reviewed

Microbial community structure in polluted Baltic Sea sediments

Edlund A, Soule T, Sjoling S, Jansson JK

Abstract

Nearly half the seabed of the Baltic Proper is incapable of supporting life of higher organisms as a consequence of oxygen depletion resulting from eutrophication. However, these areas are actually teeming with microbial life. Here we used terminal-restriction fragment length polymorphism (T-RFLP) to investigate the dominant archaeal and bacterial groups, with respect to community structure, in surface layers of bottom sediments of the Baltic Sea along a coastal pollution gradient. Both archaeal and bacterial communities formed distinct clusters along the pollution gradient and the community compositions were different at the polluted sites compared with the relatively clean reference sites. The structures of the bacterial communities were most strongly correlated to water depth, followed by organic carbon, oxygen, salinity and silicate levels. In contrast, the structures of the archaeal communities were most strongly correlated to oxygen, salinity, organic carbon, silicate and nitrate levels. Some members of the microbial communities were identified using a combination of traditional and molecular approaches. Isolates obtained on different culture media were identified by partial sequencing of their 16S rRNA genes and some novel species were found. In addition, we developed a computer program, APLAUS, to elucidate the putative identities of the most dominant community members by T-RFLP

Published in

Environmental Microbiology
2006, Volume: 8, number: 2, pages: 223-232 Publisher: BLACKWELL PUBLISHING

    UKÄ Subject classification

    Economics and Business
    Fish and Aquacultural Science
    Environmental Sciences related to Agriculture and Land-use

    Publication identifier

    DOI: https://doi.org/10.1111/j.1462-2920.2005.00887.x

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/8006