Walker, William
- Department of Plant Protection Biology, Swedish University of Agricultural Sciences
Abstract
The sense of smell is critical for the survival of insects, by as insects detect odor signals in the environment and make appropriate behavioral responses such as host preference, mate choice, and oviposition site selection. The antenna is the main olfactory organ in insects. Multiple antennal proteins have been suggested to be involved in olfactory signal transduction pathway such as odorant receptors (ORs), ionotropic receptors (IRs), odorant binding proteins (OBPs), chemosensory proteins (CSPs) and sensory neuron membrane proteins (SNMPs). In this study, we identified several olfactory gene subfamilies in the economically important Coleopteran agricultural pest,Leptinotarsa decemlineata, by assembling the adult male and female antennal transcriptomes. In the male and female antennal transcriptome, we identified a total of 37 OR genes, 10 IR genes, 26 OBP genes, 15 CSP genes, and 3 SNMP genes. Further, expression of all candidate ORs was validated in male or female antenna by semi-quantitative reverse transcription PCR. Most of the candidate OR genes have similar expression levels in male and female. A few OR genes have been detected to have male-specific (LdecOR6) or male-biased (LdecOR5, LdecOR12, LdecOR26, andLdecOR32) expression. Additionally, two OR genes (LdecOR3andLdecOR29) were observed to be expressed higher in female. Our findings make it possible for future research of the olfactory system ofL. decemlineataat the molecular level.
Keywords
transcriptome; olfactory gene; Leptinotarsa decemlineata; antenna; RT-PCR
Published in
Frontiers in Ecology and Evolution
2015, volume: 3, article number: 60
SLU Authors
UKÄ Subject classification
Zoology
Bioinformatics and Computational Biology (Methods development to be 10203)
Publication identifier
- DOI: https://doi.org/10.3389/fevo.2015.00060
Permanent link to this page (URI)
https://res.slu.se/id/publ/80665