Abstract

Transmission of dsRNA viruses between homo- and heterokaryotic mycelia paired on agar plates and into conidia has been studied in Heterobasidion annosum. Horizontal transmission of dsRNA occurred between both homo- and heterokaryotic isolates, as well as between isolates belonging to different intersterility groups. The proportions of vertical transmission into conidia were 3% and 55%, respectively, for the two isolates included in the study. RT-PCR of dsRNA and PCR-RFLP of mitochondrial markers were used to confirm transmission of dsRNA between the cytoplasms of different mycelia. The identity of nuclei and nuclear migration during experiments were verified using PCR-RFLP of several nuclear markers. (C) 2002 Elsevier Science (USA). All rights reserved.

Keywords

Heterobasidion annostan; mycovirus; transmission; dsRNA; mitochondrial markers; nuclear markers; basidiomycete; plant pathogen; horizontal transfer; vertical transfer

Published in

Fungal Genetics and Biology
2002, Volume: 36, number: 2, pages: 147-154
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE

SLU Authors

• Ihrmark, Katarina

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Stenström, Elna

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

• Stenlid, Jan

  • Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Microbiology
Other Biological Topics


Publication identifier

DOI: https://doi.org/10.1016/S1087-1845(02)00011-7

Permanent link to this page (URI)

https://res.slu.se/id/publ/80793