Abstract

The post-Golgi compartment trans-Golgi Network (TGN) is a central hub divided into multiple subdomains hosting distinct trafficking pathways, including polar delivery to apical membrane. Lipids such as sphingolipids and sterols have been implicated in polar trafficking from the TGN but the underlying mechanisms linking lipid composition to functional polar sorting at TGN subdomains remain unknown. Here we demonstrate that sphingolipids with alpha-hydroxylated acyl-chains of at least 24 carbon atoms are enriched in secretory vesicle subdomains of the TGN and are critical for de novo polar secretory sorting of the auxin carrier PIN2 to apical membrane of Arabidopsis root epithelial cells. We show that sphingolipid acyl-chain length influences the morphology and interconnections of TGN-associated secretory vesicles. Our results uncover that the sphingolipids acyl-chain length links lipid composition of TGN subdomains with polar secretory trafficking of PIN2 to apical membrane of polarized epithelial cells.

Published in

Nature Communications
2016, Volume: 7, article number: 12788
Publisher: NATURE PUBLISHING GROUP

SLU Authors

• Jonsson, Kristoffer

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

• Bhalerao, Rupali R.

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Botany


Publication identifier

DOI: https://doi.org/10.1038/ncomms12788

Permanent link to this page (URI)

https://res.slu.se/id/publ/80796