The Large Subunit rDNA Sequence of Plasmodiophora brassicae Does not Contain Intra-species Polymorphism

Abstract

Clubroot disease caused by Plasmodiophora brassicae is one of the most important diseases of cultivated brassicas. P. brassicae occurs in pathotypes which differ in the aggressiveness towards their Brassica host plants. To date no DNA based method to distinguish these pathotypes has been described. In 2011 polymorphism within the 28S rDNA of P. brassicae was reported which potentially could allow to distinguish pathotypes without the need of time-consuming bioassays. However, isolates of P. brassicae from around the world analysed in this study do not show polymorphism in their LSU rDNA sequences. The previously described polymorphism most likely derived from soil inhabiting Cercozoa more specifically Neoheteromita-like glissomonads. Here we correct the LSU rDNA sequence of P. brassicae. By using FISH we demonstrate that our newly generated sequence belongs to the causal agent of clubroot disease. (C) 2016 The Authors. Published by Elsevier GmbH.

Keywords

Clubroot; FISH (fluorescence in-situ hybridisation); LSU rDNA; pathotyping; phytomyxea; plasmodiophorids

Published in

Protist
2016, Volume: 167, number: 6, pages: 544-554

SLU Authors

• Schwelm, Arne

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    
  • University of Innsbruck

• Dixelius, Christina

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Structural Biology
Developmental Biology
Cell Biology


Publication identifier

DOI: https://doi.org/10.1016/j.protis.2016.08.008

Permanent link to this page (URI)

https://res.slu.se/id/publ/81031