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Research article2016Peer reviewedOpen access

Coetzeea brasiliensis gen. nov., sp nov isolated from larvae of Anopheles darlingi

Kaempfer, Peter; Glaeser, Stefanie P.; Marinotti, Osvaldo; Guy, Lionel; Hakansson, Sebastian; Tadei, Wanderli P.; Busse, Hans-Juergen; Terenius, Olle

Abstract

A Gram-stain-negative, rod-shaped strain, Braz8(T), isolated from larvae of Anopheles darlingi was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Braz8T was related most closely to species of the genus Thorsellia, with 95.6, 96.5 and 96.6 % similarity to the type strains of Thorsellia anophelis, Thorsellia kandunguensis and Thorsellia kenyensis, respectively, and formed a separate branch in the phylogenetic tree next to the monophyletic cluster of the genus Thorsellia. Chemotaxonomic data supported the allocation of the strain to the family Thorselliaceae. The major fatty acids were C-18 . 1 omega 7c, C-16 : 0 and C-14 : 0. The quinone system was composed of ubiquinones Q-8 and Q-7 (1 :0.3), the predominant polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the polyamine pattern showed the major compound putrescine. However, qualitative and quantitative differences in the major polyamine, polar lipid profile and fatty acid patterns distinguished strain Braz8T from species of the genus Thorsellia. Phylogenetic analysis based on 16S rRNA gene sequences, average nucleotide identity, DNA DNA hybridization, multilocus sequence analysis as well as physiological and biochemical tests distinguished strain Braz8(T) both genotypically and phenotypically from the three Thorsellia species but also showed its placement in the family Thorselliaceae. Thus, strain Braz8(T) is considered to represent a novel species of a new genus most closely related to the genus Thorsellia, for which the name Coetzeea brasiliensis gen. nov., sp. nov. is proposed. The type strain of Coetzeea brasiliensis is Braz8(T) (=LMG 29552(T)=CIP 111088(T)).

Keywords

Coetzeea brasiliensis, Anopheles darling, Thorsellia, microbiological systematics

Published in

International Journal of Systematic and Evolutionary Microbiology
2016, Volume: 66, number: 12, pages: 5211-5217
Publisher: MICROBIOLOGY SOC

      SLU Authors

      • UKÄ Subject classification

      Microbiology
      Biological Systematics

      Publication identifier

      DOI: https://doi.org/10.1099/ijsem.0.001497

      Permanent link to this page (URI)

      https://res.slu.se/id/publ/81035