Metabolic signatures altered by in vitro temperature stress in Ajuga bracteosa Wall. ex. Benth

Abstract

To elucidate how biosynthesis of plant metabolites is affected by temperature, metabolite profiles from in vitro regenerated plants raised under different temperature regimes of 10, 15 C, 20 C, 25 C and 30 C were obtained using electrospray ionization mass spectrometry (ESI-MS), and principal component analysis (PCA) was carried out to identify key metabolites. Several bin masses were detected by PCA loading scatter plots which separated the samples. In-house bin program selectively manifested the putative known metabolites depending on % total ions count and intensity of selected bins in the plant samples. Total phenolic and flavonoid content were harvested to highest levels (12.9 mg GAE/g DW and 9.3 mg QE/g DW), respectively, at 15 C. Besides, pinoresinol (lignan), some of the vital amino acids such as serine, methionine, histidine and glutamine were found to be at higher amount in plants raised at 15 C. Significant phenylpropanoids like cinnamic acid, caffeic acid and quercitol were detected at a higher concentration in plants raised at 15 C as compared to other treatments. However, phosphoenolpyruvate, and oxalosuccinate (intermediates of the pentose phosphate pathway) were accumulated the most in plants raised at 30 C and they were detected with lowest values at 10 C. Glucose and deoxy-xylose 5 phosphate (intermediates of TCA cycle) were found in higher amounts at temperature treatments of 15 and 25 C, respectively. We conclude that a low-temperature treatment (15 C) results in a stress-induced accumulation of a variety of pharmacologically important secondary metabolites.

Keywords

Ajuga; Bin masses; Electrospray ionization; Metabolite; Temperature stress

Published in

Acta Physiologiae Plantarum
2017, volume: 39, number: 4, article number: 97
Publisher: SPRINGER HEIDELBERG

SLU Authors

• Khan Kayani, Waqas

  • Department of Plant Breeding, Swedish University of Agricultural Sciences
    
  • Quaid-i-Azam University

UKÄ Subject classification

Agricultural Science

Publication identifier

• DOI: https://doi.org/10.1007/s11738-017-2394-9

Permanent link to this page (URI)

https://res.slu.se/id/publ/82564