Effect of Single Layer Centrifugation on reactive oxygen species and sperm mitochondrial membrane potential in cooled stallion semen

Abstract

Additional means are needed for evaluating the quality of stallion spermatozoa in semen doses for AI. Mitochondrial membrane potential ((m)) has been linked to fertility in some species, but is rarely used in the evaluation of cooled stallion semen; metabolic activity may be associated with reactive oxygen species production (ROS). In the present study, (m) and ROS production were measured in doses of cooled stallion semen. The effect of colloid centrifugation on these parameters was also investigated. In this case, colloid centrifugation involves centrifuging a sperm sample through a silane-coated silica colloid formulation to retrieve the most robust spermatozoa. High and low (m) in cooled stallion semen varied between stallions and between ejaculates, but was not affected by single-layer centrifugation (SLC). The SLC-selected spermatozoa produced significantly less hydrogen peroxide than controls (P

Keywords

Androcoll

Published in

Reproduction, Fertility and Development
2017, volume: 29, number: 5, pages: 1039-1045
Publisher: CSIRO PUBLISHING

SLU Authors

• Morrell, Jane

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Lagerqvist, Anne Hanna Elin

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Humblot, Patrice

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Johannisson, Anders

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Clinical Science

Publication identifier

• DOI: https://doi.org/10.1071/RD15440

Permanent link to this page (URI)

https://res.slu.se/id/publ/82569