Abstract

Although chromosomal replication is an essential feature of the bacterial life cycle, the replication mechanism and involved molecular players have never been properly characterized in the Acetobacter genera. Thanks to whole-genome sequencing, the unknown replication proteins from Acetobacter pasteurianus and Acetobacter orleanensis, DnaA-like and DnaB-like, could be identified. Despite the low nucleotide or amino acid similarity to the respective orthologs from Escherichia coli, their involvement during replication regulation was corroborated by artificial microRNA. In the Acetobacter genome, a novel replication origin, oriAo, was detected with three 9-nucleotide-long DnaA boxes to which DnaA-like proteins bind actively. Bacterial two-hybrid systems and co-immunoprecipitation confirmed the homologous and heterologous interactions between DnaA-like and DnaB-like proteins with their E. coli orthologs. This communication is due to the conserved tryptophan at position 6 for E. coli or 25 for Acetobacter that unables DnaA-like proteins to form oligomeric protein structures after its substitution. Altogether, these results provide novel insights into the genome replication mechanism in Acetobacter. (C) 2016 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Keywords

Acetobacter; DnaA protein; DnaB protein; Protein structure; Protein-protein interaction

Published in

Research in Microbiology
2016, Volume: 167, number: 8, pages: 655-668
Publisher: ELSEVIER SCIENCE BV

SLU Authors

• Grones, Peter

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Microbiology


Publication Identifiers

DOI: https://doi.org/10.1016/j.resmic.2016.06.010

Permanent link to this page (URI)

https://res.slu.se/id/publ/82871