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Research article2003Peer reviewedOpen access

The F-Box protein Skp2 participates in c-Myc protelosornal degradation and acts as a cofactor for c-Myc-regulated transcription

von, der, Lehr, N; Johansson, S; Wu, SQ; Bahram, F; Castell, A; Cetinkaya, C; Hydbring, P; Weidung, I; Nakayama, K; Nakayama, KI; Soderberg, O; Kerppola, TK; Larsson, LG

Abstract

The transcription regulatory oncoprotein c-Myc controls genes involved in cell growth, apoptosis, and oncogenesis. c-Myc is turned over very quickly through the ubiquitin/proteasome pathway. The proteins involved in this process are still unknown. We have found that Skp2 interacts with c-Myc and participates in its ubiquitylation and degradation. The interaction between Skp2 and c-Myc occurs during the G1 to S phase transition of the cell cycle in normal lymphocytes. Surprisingly, Skp2 enhances c-Myc-induced S phase transition and activates c-Myc target genes in a Myc-dependent manner. Further, Myc-induced transcription was shown to be Skp2 dependent, suggesting interdependence between c-Myc and Skp2 in activation of transcription. Moreover, Myc-dependent association of Skp2, ubiquitylated proteins, and subunits of the proteasome to a c-Myc target promoter was demonstrated in vivo. The results suggest that Skp2 is a transcriptional cofactor for c-Myc and indicates a close relationship between transcription activation and transcription factor ubiquitination

Published in

Molecular Cell
2003, volume: 11, number: 5, pages: 1189-1200
Publisher: CELL PRESS

SLU Authors

Publication identifier

  • DOI: https://doi.org/10.1016/S1097-2765(03)00193-X

Permanent link to this page (URI)

https://res.slu.se/id/publ/830