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Research article - Peer-reviewed, 2016

Optimizing the design of a reproduction toxicity test with the pond snail Lymnaea stagnalis

Charles, Sandrine; Ducrot, Virginie; Azam, Didier; Benstead, Rachel; Brettschneider, Denise; De Schamphelaere, Karel; Goncalves, Sandra Filipe; Green, John W.; Holbech, Henrik; Hutchinson, Thomas H.; Faber, Daniel; Laranjeiro, Filipe; Matthiessen, Peter; Norrgren, Leif; Oehlmann, Joerg; Reategui-Zirena, Evelyn; Seeland-Fremer, Anne; Teigeler, Matthias; Thome, Jean-Pierre; Kaplon, Marysia Tobor;
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Abstract

This paper presents the results from two ring-tests addressing the feasibility, robustness and reproducibility of a reproduction toxicity test with the freshwater gastropod Lymnaea stagnalis (RENILYS strain). Sixteen laboratories (from inexperienced to expert laboratories in mollusc testing) from nine countries participated in these ring-tests. Survival and reproduction were evaluated in L. stagnalis exposed to cadmium, tributyltin, prochloraz and trenbolone according to an OECD draft Test Guideline. In total, 49 datasets were analysed to assess the practicability of the proposed experimental protocol, and to estimate the between-laboratory reproducibility of toxicity endpoint values. The statistical analysis of count data (number of clutches or eggs per individual-day) leading to ECx estimation was specifically developed and automated through a free web-interface. Based on a complementary statistical analysis, the optimal test duration was established and the most sensitive and cost-effective reproduction toxicity endpoint was identified, to be used as the core endpoint. This validation process and the resulting optimized protocol were used to consolidate the OECD Test Guideline for the evaluation of reproductive effects of chemicals in L stagnalis. (C) 2016 Elsevier Inc. All rights reserved.

Keywords

Mollusc; Fecundity; ECx; Count data; Test design optimization

Published in

Regulatory Toxicology and Pharmacology
2016, Volume: 81, pages: 47-56
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE

    Sustainable Development Goals

    SDG6 Clean water and sanitation

    UKÄ Subject classification

    Pharmacology and Toxicology

    Publication identifier

    DOI: https://doi.org/10.1016/j.yrtph.2016.07.012

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/83052