A fibronectin mimetic motif improves integrin mediated cell biding to recombinant spider silk matrices

Abstract

The cell binding motif RGD is the most widely used peptide to improve cell binding properties of various biomaterials, including recombinant spider silk. In this paper we use genetic engineering to further enhance the cell supportive capacity of spider silk by presenting the RGD motif as a turn loop, similar to the one found in fibronectin (FN), but in the silk stabilized by cysteines, and therefore denoted FNCC. Human primary cells cultured on FNCC-silk showed increased attachment, spreading, stress fiber formation and focal adhesions, not only compared to RGD-silk, but also to silk fused with linear controls of the RGD containing motif from fibronectin. Cell binding to FNCC-silk was shown to involve the alpha 5 beta 1 integrin, and to support proliferation and migration of keratinocytes. The FNCC-silk protein allowed efficient assembly, and could even be transformed into free standing films, on which keratinocytes could readily form a monolayer culture. The results hold promise for future applications within tissue engineering. (C) 2015 Elsevier Ltd. All rights reserved.

Keywords

RGD; Cell adhesion; Disulphide bridged loop; Wound healing

Published in

Biomaterials
2016, Volume: 74, pages: 256-266 Publisher: ELSEVIER SCI LTD

SLU Authors

• Hedhammar, My

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    
  • Royal Institute of Technology (KTH)

UKÄ Subject classification

Bio Materials


Publication identifier

DOI: https://doi.org/10.1016/j.biomaterials.2015.10.013

Permanent link to this page (URI)

https://res.slu.se/id/publ/83218