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Research article2017Peer reviewedOpen access

Phylogenomic analysis of the complete sequence of a gastroenteritis-associated cetacean adenovirus (bottlenose dolphin adenovirus 1) reveals a high degree of genetic divergence

Malmberg, Maja; Rubio-Guerri, Consuelo; Hayer, Juliette; Garcia-Parraga, Daniel; Nieto-Pelegrin, Elvira; Melero, Mar; Alvaro, Teresa; Valls, Monica; Manuel Sanchez-Vizcaino, Jose; Belak, Sandor; Granberg, Fredrik

Abstract

Adenoviruses are common pathogens in vertebrates, infecting a wide range of hosts, but only having rarely been detected and correlated with disease in cetaceans. This article describes the first complete genomic sequence of a cetacean adenovirus, bottlenose dolphin adenovirus 1 (BdAdV-1), detected in captive bottlenose dolphin population ( Tursiops truncatus) suffering from self-limiting gastroenteritis. The complete genome sequence of BdAdV-1 was recovered from data generated by high-throughput sequencing and validated by Sanger sequencing. The genome is 34,080 bp long and has 220 nucleotides long inverted terminal repeats. A total of 29 coding sequences were identified, 26 of which were functionally annotated. Among the unusual features of this genome is a remarkably long 4380 bp E3 ORF1, that displays no sequence homology with the corresponding E3 regions of other adenoviruses. In addition, the fiber protein only has 26% identity with fiber proteins described in other adenoviruses. Three hypothetical proteins were predicted. The phylogenetic analysis indicates that the closest known relative to BdAdV-1 is an adenovirus detected in bottlenose dolphin (KR024710), with an amino acid sequence identity between 36 and 79% depending on the protein. Based on the phylogenic analysis, the BdAdV-1 appears to have co-evolved with its host.The results indicate that BdAdV-1 belongs to the Mastadenovirus genus of the Adenoviridae family, however, it is clearly different from other adenoviruses, especially in the 3'-end of the viral genome. The high degree of sequence divergence suggests that BdAdV-1 should be considered as a novel species in the Mastadenovirus genus. The study also demonstrates the usefulness of high-throughput sequencing to obtain full-length genomes of genetically divergent viruses. (C) 2017 The Authors. Published by Elsevier B.V.

Keywords

Adenovirus Complete genome; E3 region; Bottlenose dolphin; Tursiops truncatus; High-throughput sequencing; Viral metagenomics

Published in

Infection, Genetics and Evolution
2017, Volume: 53, pages: 47-55 Publisher: Elsevier {BV}

      SLU Authors

    • Malmberg, Maja

      • Department of Animal Biosciences, Swedish University of Agricultural Sciences
      • World Organization for Animal Health (OIE) Collaborating Centre for the Biotechnology-based Diagnosis of Infectious Diseases in Veterinary Medicine
        • Belak, Sandor

          • Department of Animal Biosciences, Swedish University of Agricultural Sciences
          • World Organization for Animal Health (OIE) Collaborating Centre for the Biotechnology-based Diagnosis of Infectious Diseases in Veterinary Medicine
        • UKÄ Subject classification

          Cell and Molecular Biology
          Pathobiology
          Medical Bioscience

          Publication identifier

          DOI: https://doi.org/10.1016/j.meegid.2017.05.008

          Permanent link to this page (URI)

          https://res.slu.se/id/publ/83595