A single carbocyclic nucleotide substitution in a 12mer DNA gives a Hoogsteen basepaired duplex (till 38 degrees C) and a hairpin (till 65 degrees C). A 600 MHZ NMR spectroscopic study.

Abstract

The impact of intramolecular stereoelectronic effects has been examined by comparison of the solution structures of natural oligo-DNA duplex, 5'((1)C(2)G(3)C(4)G(5)A(6)A(7)T(8)T(9)C(10)G(11)C(12)G)(2)(3'), and its carbocyclic-nucleotide analogues in which the pentose sugar in 2'-dA residue is replaced with its carbocyclic counterpart (i.e. 2'-deoxyaristeromycin). Based on the NMR evidences, it has been shown, that 2'-deoxyaristeromycin analog exists in a dynamic equilibrium between the two forms of duplexes, one with W-C bp and the second with Hoogsteen bp in ca 1:1 ratio at lower temperature (below 35 degrees C) and as hairpin at higher temperature (from similar to 40 degrees - 60 degrees C).

Published in

Nucleosides And Nucleotides
1999, volume: 18, number: 6-7, pages: 1593-1596
Publisher: MARCEL DEKKER INC

SLU Authors

• Agback, Peter

  • Uppsala University

UKÄ Subject classification

Structural Biology

Publication identifier

• DOI: https://doi.org/10.1080/07328319908044793

Permanent link to this page (URI)

https://res.slu.se/id/publ/83878