Abstract

The nucleobase-protected partially-deuterated 5'-O-DMTr-2'(#),2 ''(#)3',4'(#),5',5 ''-H-2(5)-2'-deoxyribonucleoside 3'-phosphoramidite phosphoramidite derivatives 17a,b - 20a,b, containing C2' isotopomeric mixture of deuterons and protons [similar to 15 atom % H-2 at C2'(R) similar to 85 atom % H-2 at C2'(S),; 65 atom % H-2 at C4'(#), > 97 atom % H-2 at C3' and C5', i.e. Blocks B and C in Fig. 1], have been site specifically incorporated into a self-complementary 12-mer [d(C(5)G(6)C(7*)G(8)A(9*)A(10)T(11) T(12*)C(13)G(14*)C(15)G(16))](2) (1) and a 20-mer [d(C(1)G(2)C(3)G(4)C(5*)G(6*)C(7*)G(8*)A(9*)A(10*)T(11*)T(12*)C(13*)G(14*)C(15*)G(16*) 9* C(17)G(18)C(19)G(20))](2) (II) DNA duplex (N* indicates the partially-deuterated blocks B and C in Fig. 1) by the solid phase synthesis methodology to develop the ''NMR window II'' concept (for our ''NMR window I'' concept see refs 5-8). The present ''NMR window II'' concept simplifies spectral crowding as well as allows the retrieval of both J-coupling and nOe informations from the partially-deuterated nucleotide residues, whereas our older ''NMR-window I'' concept helped to suppress the unwanted proton resonances by substituting with deuterium but the sensitivity of the proton resonances in the ''NMR-window'' was poorer in a 20-mer DNA duplex because of the line-broadening. The overall spectral simplification of the spectral crowding in ''NMR window II'' concept, owing to greater than or equal to 97% suppression of the proton resonances from C3' and C5', has resulted in an enhancement of the spectral resolution, and thereby following structural information could be obtained in an unambiguous manner: (i) The partial deuteration of C2' along with full suppression of H3' rersonance by deuteration creating an C2'-isotopomeric mixture has given us an unprecedented possibility for the extraction of the (3)J(H1'H2') and (3)J(H1'H2 '') coupling constant information easily and unambiguously from DQF-COSY or other double quantum experiments for the 20 base pair long DNA duplex with high accuracy as a consequence of the increased intensity of the crosspeaks because of the elimination of J(2'2 ''), J(2'3') and J(2'3') couplings in these partially-deuterated blocks (i.e. N* residues in dupler (I) and (II)]. (ii) It is also noteworthy that the T-2 relaxation for the H2 '' protons of partially-deuterated residues in deuterated duplex (I) has increased by similar to 1.5 to 2 fold compared to the nondeuterated residues (see Table 1). (iii) Because of the suppression of the proton resonances completely from C3' and C5' as well as owing to the fact that there is only similar to 15 atom % residual H-1 at C2'(S) in the beta-face, we observe only interresidual [(H2 '')(i-1)-(Ar)(i), (H1')(i-1)-(Ar)(i)] and intraesidual [(H2 ''-Ar)(i), (Ar-H1')(i), (H1'-H2 '')(i), (H4'-H1')(i), (H4'-H2 '')(i), (H4'-Ar)(i)] nOes using HAL-NOESY experiment, allowing the filtration of all proton resonances belonging to the nondeuterated nucleotides.The comparison of the relative nOe intensities, as judged by comparison of crosspeak to its own diagonal peak at the same mixing lime both in deuterated and its non-deuterated counterparts, obtained in HAL-NOESY experiment with that of a standard NOESY experiment, shows that the errors in the nOe volume estimation are quite similar, and thereby allowing the extraction of quantitative interproton distance information in ''NMR-window II'' concept. (iv) The elimination of proton resonances completely from C3' and C5' and only similar to 15 atom % residual H-1 at C2' in the beta-face has made it possible to eliminate spin-diffusion taking place through the H2'-Ar, H1'-H2', H3'-Ar, H1'-H3', H2'-H3' and H2'-H2 '' pathways in the NOESY spectra. (v) The present HAL-NOESY experiment allows an unambiguous extraction of the H1'-H4', H4'-H2 '' nOe volumes for large duplex, which are known to be quire sensitive to the sugar conformation.

Published in

Tetrahedron
1995, Volume: 51, number: 36, pages: 10065-10092 Publisher: PERGAMON-ELSEVIER SCIENCE LTD

UKÄ Subject classification

Structural Biology


Publication identifier

DOI: https://doi.org/10.1016/0040-4020(95)00579-W

Permanent link to this page (URI)

https://res.slu.se/id/publ/83884