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Research article1993Peer reviewed

The self-cleavage of lariat-RNA

Agback, Peter; Glemarec, Corine; Yin, Lee; Sandström, Anders; Plavec, Janez; Sund, Christian; Yamakage, Shun-ichi; Viswanadham, Garimella; Rousse, Bertrand; Puri, Nitin; Chattopadhyaya, Jyoti

Abstract

Lariat-RNAs 3 and 4 undergo site-specific self-cleavage reaction at the G3 --> C6/U7 phosphodiester bond by the nucleophilic attack of 2'-OH of G3 sugar moiety to its 3-phosphate to give 5'hydroxyl terminal at C6 or U7 and 2, 3-cyclic phosphodiester of G3 whereas lariat-tetramer 1, pentamer 2, the cyclic-A(2' --> 5)G-tetramer 5 and the cyclic-A(3' --> 5')G-tetramer 6 are completely stable. The lariat-RNAs 3 and 4 are the smallest RNA known to undergo self-cleavage which is reminiscent of the RNA-hammerhead (Ribozyme) activity. The geometry of the cleavage-site in 3 and 4 has been defined by full conformational analysis by NMR and molecular dynamics calculation in water.

Published in

Tetrahedron Letters
1993, volume: 34, number: 24, pages: 3929-3932
Publisher: PERGAMON-ELSEVIER SCIENCE LTD

SLU Authors

UKÄ Subject classification

Organic Chemistry

Publication identifier

  • DOI: https://doi.org/10.1016/S0040-4039(00)79266-5

Permanent link to this page (URI)

https://res.slu.se/id/publ/83902