Production of enzyme preparations on the basis of Penicillum canescens recombinant strains with a high ability for the hydrolysis of plant materials

Volkov, P. V.; Rozhkova, A. M.; Pravilnikov, A. G.; Andrianov, R. M.; Dotsenko, G. S.; Bekkarevich, A. O.; Koshelev, A. V.; Okunev, O. N.; Zorov, I. N.; Sinitsin, A. P.

doi:10.1134/S000368381201019X

Research article2012Peer reviewed

Production of enzyme preparations on the basis of Penicillum canescens recombinant strains with a high ability for the hydrolysis of plant materials

Volkov, P. V.; Rozhkova, A. M.; Pravilnikov, A. G.; Andrianov, R. M.; Dotsenko, G. S.; Bekkarevich, A. O.; Koshelev, A. V.; Okunev, O. N.; Zorov, I. N.; Sinitsin, A. P.

Abstract

An enzyme preparation has been produced on the basis of Penicillium canescens strains with the activity of cellibiohydrolase I, II; endo-1,4-beta-gluconase of Penicillium verruculosum; and beta-glucosidase of Aspergillus niger. It was shown that for the most effective hydrolysis of aspen wood pulp the optimal ratio of cellobiohydrolase and endo-1,4-beta-gluconase in enzyme preparations was 8: 2 (by protein). It was also established that the homologous xylanase secreted by the Penicillium canescens fungus is a required component for the enzyme complex for hydrolysis of the hemicellulose matrix of aspen wood.

Published in

Applied Biochemistry and Microbiology / Prikladnaya Biokhimiya i Mikrobiologiya
2012, Volume: 48, number: 1, pages: 58-64

UKÄ Subject classification

Biocatalysis and Enzyme Technology

Publication identifier

DOI: https://doi.org/10.1134/S000368381201019X

Permanent link to this page (URI)

https://res.slu.se/id/publ/84278