Two interacting PPR proteins are major Arabidopsis editing factors in plastid and mitochondria

Abstract

RNA editing is converting hundreds of cytosines into uridines during organelle gene expression of land plants. The pentatricopeptide repeat (PPR) proteins are at the core of this posttranscriptional RNA modification. Even if a PPR protein defines the editing site, a DYW domain of the same or another PPR protein is believed to catalyze the deamination. To give insight into the organelle RNA editosome, we performed tandem affinity purification of the plastidial CHLOROPLAST BIOGENESIS 19 (CLB19) PPR editing factor. Two PPR proteins, dually targeted to mitochondria and chloroplasts, were identified as potential partners of CLB19. These two proteins, a P-type PPR and a member of a small PPR-DYW subfamily, were shown to interact in yeast. Insertional mutations resulted in embryo lethality that could be rescued by embryo-specific complementation. A transcriptome analysis of these complemented plants showed major editing defects in both organelles with a very high PPR type specificity, indicating that the two proteins are core members of E+-type PPR editosomes.

Keywords

RNA editing; organelles; pentatricopeptide repeat

Published in

Proceedings of the National Academy of Sciences of the United States of America
2017, Volume: 114, number: 33, pages: 8877-8882

SLU Authors

• Lopez Obando, Mauricio

  • Department of Plant Biology, Swedish University of Agricultural Sciences
    
  • National Institute of Agricultural Research (INRA)
  • University Paris-Saclay

UKÄ Subject classification

Cell Biology
Genetics
Biochemistry and Molecular Biology


Publication identifier

DOI: https://doi.org/10.1073/pnas.1705780114

Permanent link to this page (URI)

https://res.slu.se/id/publ/84596