JMJ14, a JmjC domain protein, is required for RNA silencing and cell-to-cell movement of an RNA silencing signal in Arabidopsis

Searle, Iain R.; Pontes, Olga; Melnyk, Charles W.; Smith, Lisa M.; Baulcombe, David C.

doi:10.1101/gad.579910

Research article2010Peer reviewedOpen access

JMJ14, a JmjC domain protein, is required for RNA silencing and cell-to-cell movement of an RNA silencing signal in Arabidopsis

Searle, Iain R.; Pontes, Olga; Melnyk, Charles W.; Smith, Lisa M.; Baulcombe, David C.

Abstract

JMJ14 is a histone H3 Lys4 (H3K4) trimethyl demethylase that affects mobile RNA silencing in an Arabidopsis transgene system. It also influences CHH DNA methylation, abundance of endogenous transposon transcripts, and flowering time. JMJ14 acts at a point in RNA silencing pathways that is downstream from RNA-dependent RNA polymerase 2 (RDR2) and Argonaute 4 (AGO4). Our results illustrate a link between RNA silencing and demethylation of histone H3 trimethylysine. We propose that JMJ14 acts downstream from the Argonaute effector complex to demethylate histone H3K4 at the target of RNA silencing.

Keywords

RNA silencing; Arabidopsis; siRNAs; chromatin; histone demethylase

Published in

Genes and Development
2010, Volume: 24, number: 10, pages: 986--991
Publisher: Cold Spring Harbor Laboratory Press

UKÄ Subject classification

Genetics

Publication identifier

DOI: https://doi.org/10.1101/gad.579910

Permanent link to this page (URI)

https://res.slu.se/id/publ/84651