Xu, Wenjia
- National Institute of Agricultural Research (INRA)
Abstract
Transient expression assays are valuable techniques to study in vivo the transcriptional regulation of gene expression. These methods allow to assess the transcriptional properties of a given transcription factor (TF) or a complex of regulatory proteins against specific DNA motifs, called cis-regulatory elements. Here, we describe a fast, efficient, and reliable method based on the use of Physcomitrella patens protoplasts that allows the study of gene expression in a qualitative and quantitative manner by combining the advantage of GFP (green fluorescent protein) as a marker of promoter activity with flow cytometry for accurate measurement of fluorescence in individual cells.
Keywords
Physcomitrella patens; Protoplasts; Transient expression; Transcription factor; Subcellular localization
Published in
Methods in Molecular Biology
2016, volume: 1482, pages: 151-161
Title: Plant synthetic promoters : methods and protocols
Publisher: Humana Press
SLU Authors
UKÄ Subject classification
Agricultural Science
Publication identifier
- DOI: https://doi.org/10.1007/978-1-4939-6396-6_10
- ISBN: 978-1-4939-6394-2
- eISBN: 978-1-4939-6396-6
Permanent link to this page (URI)
https://res.slu.se/id/publ/84778