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Research article2012Peer reviewedOpen access

CrMPK3, a mitogen activated protein kinase from Catharanthus roseus and its possible role in stress induced biosynthesis of monoterpenoid indole alkaloids

Raina, Susheel Kumar; Wankhede, Dhammaprakash Pandhari; Jaggi, Monika; Singh, Pallavi; Jalmi, Siddhi Kashinath; Raghuram, Badmi; Sheikh, Arsheed Hussain; Sinha, Alok Krishna

Abstract

Background: Mitogen activated protein kinase (MAPK) cascade is an important signaling cascade that operates in stress signal transduction in plants. The biologically active monoterpenoid indole alkaloids (MIA) produced in Catharanthus roseus are known to be induced under several abiotic stress conditions such as wounding, UV-B etc. However involvement of any signaling component in the accumulation of MIAs remains poorly investigated so far. Here we report isolation of a novel abiotic stress inducible Catharanthus roseus MAPK, CrMPK3 that may have role in accumulation of MIAs in response to abiotic stress.Results: CrMPK3 expressed in bacterial system is an active kinase as it showed auto-phosphorylation and phosphorylation of Myelin Basic Protein. CrMPK3 though localized in cytoplasm, moves to nucleus upon wounding. Wounding, UV treatment and MeJA application on C. roseus leaves resulted in the transcript accumulation of CrMPK3 as well as activation of MAPK in C. roseus leaves. Immuno-precipitation followed by immunoblot analysis revealed that wounding, UV treatment and methyl jasmonate (MeJA) activate CrMPK3. Transient over-expression of CrMPK3 in C. roseus leaf tissue showed enhanced expression of key MIA biosynthesis pathway genes and also accumulation of specific MIAs.Conclusion: Results from our study suggest a possible involvement of CrMPK3 in abiotic stress signal transduction towards regulation of transcripts of key MIA biosynthetic pathway genes, regulators and accumulation of major MIAs.

Keywords

Catharanthus roseus; Methyl jasmonate; Mitogen activated protein kinase; Monoterpenoid indole alkaloid; Secondary metabolism

Published in

BMC Plant Biology
2012, volume: 12, article number: 134

SLU Authors

  • Jalmi, Siddhi

    • National Institute of Plant Genome Research

UKÄ Subject classification

Biochemistry
Environmental Sciences
Molecular Biology

Publication identifier

  • DOI: https://doi.org/10.1186/1471-2229-12-134

Permanent link to this page (URI)

https://res.slu.se/id/publ/85607