Research article - Peer-reviewed, 2005
Experimentally induced subnormal or normal luteal phases in sheep: reproductive hormone profiles and uterine sex steroid receptor expression
Tasende C, Forsberg M, Rodriguez-Pinon M, Acuna S, Garofalo EGAbstract
This study investigated if ewes expected to have subnormal luteal phases (SNLP) present a different pattern of uterine oestrogen receptor ( ER) and progesterone receptor ( PR) expression at the expected time of premature luteolysis. The concentrations of uterine ER, PR and ER alpha mRNA, and the steroid ovarian hormone were determined in anoestrous ewes treated with either gonadotrophin-releasing hormone ( GnRH) to develop a SNLP (n=16), or progesterone+ GnRH to develop a normal luteal phase (NLP; n=16). The ER, PR and ERa mRNA concentrations were measured using binding and solution hybridisation assays, while the hormone level concentration was measured by radioimmunoassay. In all ewes, a luteinising hormone- and follicle-stimulating hormone- synchronised surge was found. The SNLP group had lower preovulatory oestradiol levels than the NLP group. On Day 5, the SNLP group had lower progesterone levels, and higher uterine ER, PR and ERa mRNA concentrations than the NLP group. While in the SNLP group the receptor expression increased from Days 1 to 5, in the NLP group the receptor expression decreased. The results suggest that the induction of steroid receptor expression in the uterus and the hormonal environment found in the experimental SNLP group at the expected time of premature luteolysis may be involved in the mechanisms causing SNLPPublished in
Reproduction, Fertility and Development2005, volume: 17, number: 5, pages: 565-571
Publisher: C S I R O PUBLISHING
Authors' information
Rodríguez-Piñon, M
Garófalo, E.G.
Acuña, S
Forsberg, Mats
Swedish University of Agricultural Sciences, Department of Biomedical Science and Veterinary Public Health
Tasende, Celia
Swedish University of Agricultural Sciences, Department of Biomedical Science and Veterinary Public Health
UKÄ Subject classification
Veterinary Science
Animal and Dairy Science
Publication Identifiers
DOI: https://doi.org/10.1071/RD05001
URI (permanent link to this page)
https://res.slu.se/id/publ/8587