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Research article2013Peer reviewed

In vivo effect confirmation of anti-androgenic compounds in sediment contact tests with Potamopyrgus antipodarum

Schmitt, Claudia; LaMoree, Marja; Leonards, Pim; Weiss, Jana M.; de Deckere, Eric

Abstract

In order to investigate the sensitivity of Potamopyrgus antipodarum to anti-androgenic compounds, three spiked sediment tests were performed. The substances benzanthrone (7H-benz[de]anthracen-7-one), traseolide (ATII) and androstenone (5-Androst-16-en-3-one) were previously identified in an effect-directed analysis study of the river Schijn in the north of Belgium. Although, in previous studies, all of the three compounds exhibited anti-androgenic activities in vitro, only the oxy-PAH benzanthrone had significant stimulating effects on the snails' reproduction. The reproduction of P. antipodarum was significantly stimulated, following a sigmoidal dose response curve, whereby an EC50 of 10ng/g dry sediment was calculated. Mortality was significantly increased at the highest concentration (69ng/g dry sediment). The results indicate different relative potencies for the in vivo test with P. antipodarum and the in vitro anti-AR-CALUX assay, performed in a previous study. This highlights the importance of combined in vitro and in vivo assays for the effect assessment of field sediments.

Keywords

Traseolide; androstenone; benzanthrone; reproduction; Prosobranch snails

Published in

Journal of Environmental Science and Health, Part A
2013, Volume: 48, number: 5, pages: 475-480 Publisher: TAYLOR & FRANCIS INC

    UKÄ Subject classification

    Analytical Chemistry

    Publication identifier

    DOI: https://doi.org/10.1080/10934529.2013.730387

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/86596