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Research article - Peer-reviewed, 2018

Effect of bovine seminal plasma on bovine endometrial epithelial cells in culture

Nongbua, T.; Guo, Y.; Edman, A.; Humblot, P.; Morrell, J. M.

Abstract

The purpose of this study was to investigate the effect of seminal plasma (SP) from bulls of known fertility on bovine endometrial epithelial cells (bEEC) in culture. The bEEC from passage 5, approximately 5.0-13x10(5)cells per flask, were challenged with SP from bulls of high or low fertility (n=3 and 2, respectively) or PBS (control), at 1% (75 l) or 4% (300 mu l) and were incubated for 72hr (n=13 per challenge). Total cell number and viability of bEEC after challenge with 1% SP from either high- or low-fertility bulls (75H or 75L, respectively) did not differ from controls. In contrast, challenge with 4% of SP from high- or low-fertility bulls (300H or 300L) negatively affected bEEC cell number and viability. Challenge with 300L had a greater adverse effect than 300H. These results suggest that the negative effect of bovine SP on bEEC is both dose-dependent and fertility-dependent.

Published in

Reproduction in Domestic Animals
2018, Volume: 53, number: 1, pages: 85-92