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Research article2014Peer reviewed

The use of liquid chromatography tandem mass spectrometry to detect proteins in saliva from horses with and without systemic inflammation

Jacobsen, Stine; Adler, Ditte Marie Top; Bundgaard, Louise; Sorensen, Mette Aamand; Andersen, Pia Haubro; Bendixen, Ernoke

Abstract

The objective of the study was to assess global expression of proteins in equine saliva using liquid chromatography tandem mass spectrometry (LC-MS/MS). Saliva was obtained from seven horses with and six horses without evidence of systemic inflammatory disease. Tryptic peptides from saliva were analysed by LC-MS/MS.Of 195 unique proteins identified, 57 were detected only in saliva samples from horses with systemic inflammation (in two to six of the seven horses). Among the differentially expressed proteins were several acute phase proteins (APPs) such as serum amyloid A, fibrinogen, haptoglobin, and alphal-acid glycoprotein.The study is the first to describe detection of inflammatory proteins in horse saliva. The proteins detected were similar to those described in saliva from cattle, small ruminants and pigs. Detection of APPs in horses with systemic inflammation suggests that saliva may be used for non-invasive disease monitoring in horses as in humans, pigs and dogs. (C) 2014 Elsevier Ltd. All rights reserved.

Keywords

Equine; Inflammation; Mass spectrometry; Proteomics; Saliva

Published in

Veterinary Journal
2014, Volume: 202, number: 3, pages: 483-488 Publisher: ELSEVIER SCI LTD

    UKÄ Subject classification

    Medical Bioscience

    Publication identifier

    DOI: https://doi.org/10.1016/j.tvjl.2014.08.032

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/87156