Zhang, Bo
- Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
Research article2017Peer reviewedOpen access
Zhang, Bo; Holmlund, Mattias; Lorrain, Severine; Norberg, Mikael; Bako, Laszlo; Fankhauser, Christian; Nilsson, Ove
Both light and temperature have dramatic effects on plant development. Phytochrome photoreceptors regulate plant responses to the environment in large part by controlling the abundance of PHYTOCHROME INTERACTING FACTOR (PIF) transcription factors. However, the molecular determinants of this essential signaling mechanism still remain largely unknown. Here, we present evidence that the BLADE-ON-PETIOLE (BOP) genes, which have previously been shown to control leaf and flower development in Arabidopsis, are involved in controlling the abundance of PIF4. Genetic analysis shows that BOP2 promotes photo-morphogenesis and modulates thermomorphogenesis by suppressing PIF4 activity, through a reduction in PIF4 protein level. In red-light-grown seedlings PIF4 ubiquitination was reduced in the bop2 mutant. Moreover, we found that BOP proteins physically interact with both PIF4 and CULLIN3A and that a CULLIN3-BOP2 complex ubiquitinates PIF4 in vitro. This shows that BOP proteins act as substrate adaptors in a CUL3(BOP1/BOP2) E3 ubiquitin ligase complex, targeting PIF4 proteins for ubiquitination and subsequent degradation.
eLife
2017, Volume: 6Publisher: ELIFE SCIENCES PUBLICATIONS LTD
Developmental Biology
DOI: https://doi.org/10.7554/eLife.26759
https://res.slu.se/id/publ/87460