Abstract

Background: Multiplex flow cytometry is in widespread use for detection of cytokines in human samples. However, no report on the measurement of porcine cytokines using this method has previously been published. We report on the detection of the porcine proinflammatory cytokines TNF-alpha, IL-8, and IL-1 beta by the xMap-assay for multiplex flow cytometry Methods: Commercially available antibodies to porcine cytokines were used as capture antibodies by attaching them to goat anti-mouse IgG coated microspheres with different fluorescent signatures. By the use of biotinylated detection antibodies and SAv-PE the amount of cytokines bound to the spheres were measured. Experiments were performed to determine the limits of detection and the amount of crossreactivity in buffer, serum, and plasma, using spiking with recombinant porcine cytokines. Results: The limit of detection ranged from 0.18 to 12 ng/ml. Generally, the detection limit was higher in serum and plasma, than in buffer. No crossreactivity between reagents was found. Conclusions: Porcine proinflammatory cytokines can be detected utilizing this method with satisfactory detection limits, and no crossreaction between the reagents involved. (c) 2006 International Society for Analytical Cytology

Published in

Cytometry Part A
2006, Volume: 69A, number: 5, pages: 391-395
Publisher: WILEY-LISS

SLU Authors

• Johannisson, Anders

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Kruse, Robert

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Dernfalk, Johanna

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Jensen Waern, Marianne

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Animal and Dairy Science
Veterinary Science


Publication identifier

DOI: https://doi.org/10.1002/cyto.a.20271

Permanent link to this page (URI)

https://res.slu.se/id/publ/8819