Abstract

Embryogenic cell lines of Norway spruce consist of a large number of somatic embryos. The cell lines have been divided into two groups, A and B. The group B embryos are developmentally blocked, Extracts of mature spruce seeds stimulate group B embryos to develop a morphology comparable to group A embryos. However, seed extract inhibits early embryo development. The active components in seed extract were shown probably to be proteins. Extracts of mature seeds contain chitinase-like proteins as recognized by an antibody towards chitinase 4 in sugar beet. Proteins of similar sizes were detected by the same antibody in the conditioned medium of group A, but not in group B. A chitinase cl-related chitinase and a nod factor had a stimulating effect on early embryo development, but did not influence the later stages of embryo development.

Keywords

Picea abies; somatic embryogenesis; embryo development; seed extract

Published in

Journal of Experimental Botany
1998, Volume: 49, number: 319, pages: 155-162

SLU Authors

• Egertsdotter, Ulrika

  • Sveriges lantbruksuniversitet, Swedish University of Agricultural Sciences
    

• Von Arnold, Sara

  • Sveriges lantbruksuniversitet, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Developmental Biology


Publication identifier

DOI: https://doi.org/10.1093/jexbot/49.319.155

Permanent link to this page (URI)

https://res.slu.se/id/publ/88503