Abstract

Plant hormones are a group of naturally occurring, low-abundance organic compounds that influence physiological processes in plants. Our knowledge of the distribution profiles of phytohormones in plant organs, tissues, and cells is still incomplete, but advances in mass spectrometry have enabled significant progress in tissue-and cell-type-specific analyses of phytohormones over the last decade. Mass spectrometry is able to simultaneously identify and quantify hormones and their related substances. Biosensors, on the other hand, offer continuous monitoring; can visualize local distributions and real-time quantification; and, in the case of genetically encoded biosensors, are noninvasive. Thus, biosensors offer additional, complementary technologies for determining temporal and spatial changes in phytohormone concentrations. In this review, we focus on recent advances in mass spectrometry-based quantification, describe monitoring systems based on biosensors, and discuss validations of the various methods before looking ahead at future developments for both approaches.

Keywords

phytohormone; mass spectrometry; biosensor; cell biology; resolution; sensitivity

Published in

Annual Review of Plant Biology
2017, Volume: 68, pages: 323-348

SLU Authors

• Novak, Ondrej

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    
  • Palacký University Olomouc

• Ljung, Karin

  • Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Cell Biology


Publication identifier

DOI: https://doi.org/10.1146/annurev-arplant-042916-040812

Permanent link to this page (URI)

https://res.slu.se/id/publ/89043