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Research article2001Peer reviewedOpen access

Heat stress response in pea involves interaction of mitochondrial nucleoside diphosphate kinase with a novel 86-kilodalton protein

Galvis, MLE; Marttila, S; Hakansson, G; Forsberg, J; Knorpp, C

Abstract

In this work we have further characterized the first mitochondrial nucleoside diphosphate kinase (mtNDPK) isolated from plants. The mitochondrial isoform was found to be especially abundant in reproductive and young tissues. Expression of the pea (Pisum sativum L. cv Oregon sugarpod) mtNDPK was not affected by different stress conditions. However, the pea mtNDPK was found to interact with a novel 86-kD protein, which is de novo synthesized in pea leaves upon exposure to heat. Thus, we have evidence for the involvement of mtNDPK in mitochondrial heat response in pea in vivo. Studies on oligomerization revealed that mtNDPK was found in complexes of various sizes, corresponding to the sizes of e.g. hexamers, tetramers, and dimers, indicating flexibility in oligomerization. This flexibility, also found for other NDPK isoforms, has been correlated with the ability of this enzyme to interact with other proteins. We believe that the mtNDPK is involved in heat stress response in pea, possibly as a modulator of the 86-kD protein.

Published in

Plant Physiology
2001, Volume: 126, number: 1, pages: 69-77

      SLU Authors

    • Marttila, Salla

      • Department of Crop Science, Swedish University of Agricultural Sciences

    UKÄ Subject classification

    Cell Biology

    Publication identifier

    DOI: https://doi.org/10.1104/pp.126.1.69

    Permanent link to this page (URI)

    https://res.slu.se/id/publ/90456