Research article2009Peer reviewed
Development and characterization of microsatellite markers in the fungus Antrodia cinnamomea from dbEST database
Kanagarajan, Selvaraju; Muthusamy, Saraladevi; Chen, Emily Chin-Fun; Tsay, Hsin-Sheng
Abstract
We developed and characterized 8 polymorphic microsatellite loci or simple sequence repeats from the expressed sequence tags database of the medicinal fungus, Antrodia cinnamomea. Variability was assessed in a panel of 23 strains collected from various regions of Taiwan. The number of alleles per polymorphic locus ranged from two to seven loci with an average of 4.375 alleles per locus. The observed and expected heterozygosity values ranged from 0.130 to 0.783 and from 0.122 to 0.809, respectively. No significant Hardy-Weinberg equilibrium was detected (P < 0.01) at all loci. The EST-SSR markers developed in the present study can be used for strain identification and population genetic studies in A. cinnamomea.
Keywords
Antrodia cinnamomea; EST-SSR; Fungus; Markers; Microsatellites; Simple sequence repeats
Published in
Conservation Genetics
2009, Volume: 10, number: 5, pages: 1487-1490 Publisher: SPRINGER
UKÄ Subject classification
Evolutionary Biology
Genetics
Publication identifier
DOI: https://doi.org/10.1007/s10592-008-9765-0
Permanent link to this page (URI)
https://res.slu.se/id/publ/90708