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Research article2006Peer reviewedOpen access

Development of a rapid and simple Agrobacterium tumefaciens-mediated transformation system for the fungal pathogen Heterobasidion annosum

Samils N, Elfstrand M, Czederpiltz DLL, Fahleson J, Olson A, Dixelius C, Stenlid J

Abstract

Heterobasidion annosum causes root and butt-rot in trees and is the most serious forest pathogen in the northern hemisphere. We developed a rapid and simple Agrobacterium-mediated method of gene delivery into H. annosum to be used in functional studies of candidate genes and for visualization of mycelial interactions. Heterobasidion annosum TC 32-1 was cocultivated at pH 5.6 and 20 degrees C in Hagems medium with Agrobacterium tumefaciens C58 carrying plasmids with hygromycin B resistance as the selectable marker and green fluorescent protein as a visual marker. We obtained 18 mitotically stable transformed isolates showing green fluorescence protein activity

Keywords

Agrobacterium tumefaciens; Heterobasidion annosum; green fluorescent protein (GFP); transformation

Published in

FEMS Microbiology Letters
2006, Volume: 255, number: 1, pages: 82-88 Publisher: BLACKWELL PUBLISHING