Research article2006Peer reviewedOpen access
Genome-wide occupancy profile of mediator and the Srb8-11 module reveals interactions with coding regions
Zhu, XF; Wiren, M; Sinha, I; Rasmussen, NN; Linder, T; Holmberg, S; Ekwall, K; Gustafsson, CM
Abstract
Mediator exists in a free form containing the Med12, Med13, CDK8, and CycC subunits (the Srb8-11 module) and a smaller form, which lacks these four subunits and associates with RNA polymerase II (Pol II), forming a holoenzyme. We use chromatin immunoprecipitation (ChIP) and DNA microarrays to investigate genome-wide localization of Mediator and the Srb8-11 module in fission yeast. Mediator and the Srb8-11 module display similar binding patterns, and interactions with promoters and upstream activating sequences correlate with increased transcription activity. Unexpectedly, Mediator also interacts with the downstream coding region of many genes. These interactions display a negative bias for positions closer to the 5' ends of open reading frames (ORFs) and appear functionally important, because downregulation of transcription in a temperature-sensitive med17 mutant strain correlates with increased Mediator occupancy in the coding region. We propose that Mediator coordinates transcription initiation with transcriptional events in the coding region of eukaryotic genes.
Published in
Molecular Cell
2006, Volume: 22, number: 2, pages: 169-178 Publisher: CELL PRESS
UKÄ Subject classification
Biochemistry and Molecular Biology
Publication identifier
DOI: https://doi.org/10.1016/j.molcel.2006.03.032
Permanent link to this page (URI)
https://res.slu.se/id/publ/90850