Molecular phylogenetics of ascomycotal adhesins - A novel family of putative cell-surface adhesive proteins in fission yeasts

Abstract

In this work, we identify a family of putative adhesins in the fission yeasts Schizosaccharomyces pombe and Schizosaccharomyces japonicus. The members of this family share a conserved tandem repeat related to those found in the Candida albicans Als family of adhesins. Unlike previously characterised adhesins that possess conserved ligand-binding domains at the N-terminus, this group of proteins carry ligand-binding domains at their C-termini. We demonstrate that one such domain-the uncharacterised GLEYA domain, is related to the lectin-like ligand-binding domain found in the Saccharomyces cerevisiae Flo proteins. Unlike the Flo and Als proteins, the fission yeast adhesins do not contain detectable glycosyl phosphatidyl inositol (GPI) membrane anchor signals to mediate their attachment to the cell wall, which may suggest a novel cell wall attachment mechanism. Further sequence analysis identified several putative adhesins in the sub-phylum of Pezizomycotina, where only a few adhesins have been described to date. (C) 2007 Elsevier Inc. All rights reserved.

Keywords

adhesin; flocculation; cell wall; Schizosaccharomyces pombe; tandem repeat

Published in

Fungal Genetics and Biology
2008, Volume: 45, number: 4, pages: 485-497 Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE

UKÄ Subject classification

Bioinformatics and Systems Biology


Publication identifier

DOI: https://doi.org/10.1016/j.fgb.2007.08.002

Permanent link to this page (URI)

https://res.slu.se/id/publ/90851