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Abstract

Mediator is an evolutionary conserved coregulator complex required for transcription of almost all RNA polymerase II-dependent genes. The Schizosaccharomyces pombe Mediator consists of two dissociable componentsa core complex organized into a head and middle domain as well as the Cdk8 regulatory subcomplex. In this work we describe a functional characterization of the S. pombe Mediator. We report the identification of the S. pombe Med20 head subunit and the isolation of ts alleles of the core head subunit encoding med17. Biochemical analysis of med8(ts), med17(ts), med18, med20 and med27 alleles revealed a stepwise head domain molecular architecture. Phenotypical analysis of Cdk8 and head module alleles including expression profiling classified the Mediator mutant alleles into one of two groups. Cdk8 module mutants flocculate due to overexpression of adhesive cell-surface proteins. Head domain-associated mutants display a hyphal growth phenotype due to defective expression of factors required for cell separation regulated by transcription factor Ace2. Comparison with Saccharomyces cerevisiae Mediator expression data reveals that these functionally distinct modules are conserved between S. pombe and S. cerevisiae.

Published in

Nucleic Acids Research
2008, volume: 36, number: 8, pages: 2489-2504
Publisher: OXFORD UNIV PRESS

SLU Authors

UKÄ Subject classification

Biochemistry
Molecular Biology

Publication identifier

  • DOI: https://doi.org/10.1093/nar/gkn070

Permanent link to this page (URI)

https://res.slu.se/id/publ/90853