Andersson, Marlene
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Research article2017Peer reviewedOpen access
Mass spectrometry captures structural intermediates in protein fiber self-assembly
Landreh, Michael; Andersson, Marlene; Marklund, Erik G.; Jia, Qiupin; Meng, Qing; Johansson, Jan; Robinson, Carol V.; Rising, Anna
Abstract
Self-assembling proteins, the basis for a broad range of biological scaffolds, are challenging to study using most structural biology approaches. Here we show that mass spectrometry (MS) in combination with MD simulations captures structural features of short-lived oligomeric intermediates in spider silk formation, providing direct insights into its complex assembly process.
Published in
Chemical Communications
2017, Volume: 53, number: 23, pages: 3319-3322
Publisher: ROYAL SOC CHEMISTRY
Johansson, Jan
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
- Karolinska Institute
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
Rising, Anna
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
- Department of Animal Biosciences, Swedish University of Agricultural Sciences
SLU Authors
UKÄ Subject classification
Biochemistry and Molecular Biology
Publication identifier
DOI: https://doi.org/10.1039/c7cc00307b
Permanent link to this page (URI)
https://res.slu.se/id/publ/90938