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Research article - Peer-reviewed, 2018

A standardized toolkit for genetic engineering of CTG Glade yeasts

Defosse, Tatiana A.; Courdavault, Vincent; Coste, Alix T.; Clastre, Marc; de Bernonville, Thomas Duge; Godon, Charlotte; Vandeputte, Patrick; Lanoue, Arnaud; Touze, Antoine; Linder, Tomas; Droby, Samir; Rosa, Carlos A.; Sanglard, Dominique; d'Enfert, Christophe; Bouchara, Jean-Philippe; Giglioli-Guivarc'h, Nathalie; Papon, Nicolas


We have developed a series of synthetic constructs suitable to genetically manipulate a broad range of yeast species belonging to the fungal CTG Glade. This molecular toolbox notably allows heterologous gene expression, single or dual fluorescence labeling and construction of luciferase-expressing strains for bioluminescence imaging.


Candida; Synthetic construct; Standardization; beta-galactosidase; Fluorescent proteins; Luciferase

Published in

Journal of Microbiological Methods
2018, Volume: 144, pages: 152-156

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