Abstract

Susceptability of Ascaridia galli to benzimidazole (BZ) was investigated using faecal egg count reduction test (FECRT), in ovo larval development test (LDT) and genetic markers (mutations at codons 167, 198 and 200 of beta-tubulin gene). Six flocks (F1-F6) of a commercial laying hen farm with different number of exposure to BZ were recruited. The FECR was calculated by analyzing individual faeces (F1, F2, F4 and F5) before and 10 days after treatment. The LDT was performed on parasite eggs from pooled samples from F1 to F6 and LC50 and LC99 were calculated. DNA was extracted from 120 worms and sequenced for beta-tubulin gene. In all flocks, the FECRs were above 95% (lower CI above 90%). No significant difference was observed (p > 0.05) among obtained LC50 (F1/F4 and F2/F5 vs F3/F6) in the LDT. However, LC50 and LC99 were higher than suggested values for declaration of resistance in other nematode species. No variation was observed in codon positions involved in BZ resistance. Overall, our results indicated lack of evidence of resistance to BZ in A. galli. More research is needed to confirm these results and to further optimize the existing tools for detection and monitoring of anthelmintic resistance in A. galli.

Keywords

Ascaridia galli; Benzimidazole resistance; FECRT; LDT; Genetic markers

Published in

Parasitology
2017, Volume: 144, number: 10, pages: 1338-1345
Publisher: CAMBRIDGE UNIV PRESS

SLU Authors

• Tarbiat, Behdad

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Tyden, Eva

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Höglund, Johan

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

Associated SLU-program

AMR: Parasite
AMR: Bacteria


UKÄ Subject classification

Pathobiology


Publication identifier

DOI: https://doi.org/10.1017/S0031182017000531

Permanent link to this page (URI)

https://res.slu.se/id/publ/92709