Cytoprotective Encapsulation of Individual Jurkat T Cells within Durable TiO2 Shells for T-Cell Therapy

Abstract

Lymphocytes, such as T cells and natural killer (NK) cells, have therapeutic promise in adoptive cell transfer (ACT) therapy, where the cells are activated and expanded in vitro and then infused into a patient. However, the in vitro preservation of labile lymphocytes during transfer, manipulation, and storage has been one of the bottlenecks in the development and commercialization of therapeutic lymphocytes. Herein, we suggest a cell-in-shell (or artificial spore) strategy to enhance the cell viability in the practical settings, while maintaining biological activities for therapeutic efficacy. A durable titanium oxide (TiO2) shell is formed on individual Jurkat T cells, and the CD3 and other antigens on cell surfaces remain accessible to the antibodies. Interleukin-2 (IL-2) secretion is also not hampered by the shell formation. This work suggests a chemical toolbox for effectively preserving lymphocytes in vitro and developing the lymphocyte-based cancer immunotherapy.

Keywords

artificial spores; cytoprotection; immunology; mineralization; titanates

Published in

Angewandte Chemie International Edition
2017, volume: 56, number: 36, pages: 10702-10706
Publisher: WILEY-V C H VERLAG GMBH

SLU Authors

• Seisenbaeva, Gulaim

  • The Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences
    

• Kessler, Vadim

  • The Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences
    

Global goals (SDG)

SDG3 Good health and well-being

UKÄ Subject classification

Organic Chemistry

Publication identifier

• DOI: https://doi.org/10.1002/anie.201703886

Permanent link to this page (URI)

https://res.slu.se/id/publ/92712