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Research article - Peer-reviewed, 2017

Cytoprotective Encapsulation of Individual Jurkat T Cells within Durable TiO2 Shells for T-Cell Therapy

Youn, Wongu; Ko, Eun Hyea; Kim, Mi-Hee; Park, Matthew; Hong, Daewha; Seisenbaeva, Gulaim A.; Kessler, Vadim G.; Choi, Insung S.

Abstract

Lymphocytes, such as T cells and natural killer (NK) cells, have therapeutic promise in adoptive cell transfer (ACT) therapy, where the cells are activated and expanded in vitro and then infused into a patient. However, the in vitro preservation of labile lymphocytes during transfer, manipulation, and storage has been one of the bottlenecks in the development and commercialization of therapeutic lymphocytes. Herein, we suggest a cell-in-shell (or artificial spore) strategy to enhance the cell viability in the practical settings, while maintaining biological activities for therapeutic efficacy. A durable titanium oxide (TiO2) shell is formed on individual Jurkat T cells, and the CD3 and other antigens on cell surfaces remain accessible to the antibodies. Interleukin-2 (IL-2) secretion is also not hampered by the shell formation. This work suggests a chemical toolbox for effectively preserving lymphocytes in vitro and developing the lymphocyte-based cancer immunotherapy.

Keywords

artificial spores; cytoprotection; immunology; mineralization; titanates

Published in

Angewandte Chemie International Edition
2017, Volume: 56, number: 36, pages: 10702-10706
Publisher: WILEY-V C H VERLAG GMBH

      SLU Authors

    • Seisenbaeva, Gulaim

      • The Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences
      • Kessler, Vadim

        • The Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences

      Sustainable Development Goals

      SDG3 Good health and well-being

      UKÄ Subject classification

      Organic Chemistry

      Publication identifier

      DOI: https://doi.org/10.1002/anie.201703886

      Permanent link to this page (URI)

      https://res.slu.se/id/publ/92712