Abstract

The interactions between perfluoroalkyl substances (PFASs) and a phospholipid bilayer (1,2-dimyristoyl-sn-glycero-3-phosphocholine) were investigated at the molecular level using neutron reflectometry. Representative PFASs with different chain length and functional groups were selected in this study including: perfluorobutane sulfonate (PFBS), perfluorohexanoate (PFHxA), perfluorohexane sulfonate (PFHxS), perfluorononanoate (PFNA), perfluorooctane sulfonate (PFOS), and perfluorooctane sulfonamide (FOSA). All PFASs were found to interact with the bilayer by incorporation, indicating PFAS ability to accumulate once ingested or taken up by organisms. The interactions were observed to increase with chain length and vary with the functional group as SO2NH2(FOSA) > SO2O-(PFOS) > COO-(PFNA). The PFAS hydrophobicity, which is strongly correlated with perfluorocarbon chain length, was found to strongly influence the interactions. Longer chain PFASs showed higher tendency to penetrate into the bilayer compared to the short-chain compounds. The incorporated PFASs could for all substances but one (PFNA) be removed from the lipid membrane by gentle rinsing with water (2 mL min(-1)). Although short -chain PFASs have been suggested to be the potentially less bioaccumulative alternative, we found that in high enough concentrations they can also disturb the bilayer. The roughness and disorder of the bilayer was observed to increase as the concentration of PFASs increased (in particular for the high concentrations of short-chain substances i.e. PFHxA and PFBS), which can be an indication of aggregation of PFASs in the bilayer. (C) 2017 Elsevier Inc. All rights reserved.

Keywords

PFSAs; PFHxA; PFASs; PFBS; PFHxS; PFOS; PFNA; PFCAs; FOSA; DMPC; Neutron reflectometry

Published in

Journal of Colloid and Interface Science
2018, Volume: 511, pages: 474-481

SLU Authors

• Ahrens, Lutz

  • Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences
    

• de Campos Pereria, Hugo

  • Department of Soil and Environment, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Environmental Sciences
Organic Chemistry


Publication identifier

DOI: https://doi.org/10.1016/j.jcis.2017.09.102

Permanent link to this page (URI)

https://res.slu.se/id/publ/92726