Research article - Peer-reviewed, 2017
BatchMap: A parallel implementation of the OneMap R package for fast computation of F-1 linkage maps in outcrossing speciesSchiffthaler, Bastian; Bernhardsson, Carolina; Ingvarsson, Par K.; Street, Nathaniel R.
AbstractWith the rapid advancement of high throughput sequencing, large numbers of genetic markers can be readily and cheaply acquired, but most current software packages for genetic map construction cannot handle such dense input. Modern computer architectures and server farms represent untapped resources that can be used to enable higher marker densities to be processed in tractable time. Here we present a pipeline using a modified version of OneMap that parallelizes over bottleneck functions and achieves substantial speedups for producing a high density linkage map (N = 20,000). Using simulated data we show that the outcome is as accurate as the traditional pipeline. We further demonstrate that there is a direct relationship between the number of markers used and the level of deviation between true and estimated order, which in turn impacts the final size of a genetic map.
Keywordscomputing; gene sequencing
Published inPLoS ONE
2017, volume: 12, number: 12, article number: e0189256
Publisher: PUBLIC LIBRARY SCIENCE
Street, Nathaniel R.
UKÄ Subject classification
Bioinformatics and Systems Biology
URI (permanent link to this page)