Abstract

Traditionally, extenders for bull semen included egg yolk or milk, but recently there has been a move to avoid material of animal origin. The aim of this study was to evaluate the effects of two commercial extenders (based on soya lecithin and liposomes) on bull sperm quality after cryopreservation. Post-thaw sperm quality was evaluated by computer-assisted sperm analysis and flow cytometric assessment of membrane integrity, chromatin integrity, mitochondrial membrane potential, production of reactive oxygen species and tyrosine phosphorylation. Furthermore, an artificial insemination (AI) trial was conducted, and 56-day non-return rates were evaluated. Semen frozen in the liposome-based extender showed similar membrane integrity and higher mitochondrial membrane potential compared to those in the soya lecithin-based extender. Chromatin integrity and production of live H2O2+ reactive oxygen species were similar in both extenders. Less superoxide was produced in the samples extended with liposome-based extender, with or without menadione stimulation. Chromatin integrity and tyrosine phosphorylation were not affected by either type of extender. No differences in 56-day non-return rate between extenders containing soya lecithin and liposomes were observed in the AI trial (66%+/- 0.8 and 65%+/- 0.8, respectively). In conclusion, the sperm quality of bull semen frozen in the two extenders that do not contain material of animal origin was similar, although the semen frozen in the liposome-based extender had higher mitochondrial membrane potential. Either extender could be used in situations where extenders containing material of animal origin are to be avoided.

Published in

Reproduction in Domestic Animals
2018, Volume: 53, number: 1, pages: 127-136
Publisher: WILEY

SLU Authors

• Bezerra Lima Verde, Isabel

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    
  • Universidade Tiradentes

• Johannisson, Anders

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Ntallaris, Theodoros

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

• Al-Essawe, Essraa M

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    
  • Al-Nahrain University

• Al-Kass, Ziyad

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    
  • University of Mosul

• Nongbua, Thanapol

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    
  • Mahasarakham University

• Lundeheim, Nils

  • Department of Animal Biosciences, Swedish University of Agricultural Sciences
    

• Morrell, Jane

  • Department of Clinical Sciences, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Animal and Dairy Science


Publication identifier

DOI: https://doi.org/10.1111/rda.13080

Permanent link to this page (URI)

https://res.slu.se/id/publ/93677