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Research article2018Peer reviewedOpen access

Formulation and stabilization of an Arthrobacter strain with good storage stability and 4-chlorophenol-degradation activity for bioremediation

Bjerketorp, Joakim; Roling, Wilfred F. M.; Feng, Xin-Mei; Garcia, Armando Hernandez; Heipieper, Hermann J.; Hakansson, Sebastian

Abstract

Chlorophenols are widespread and of environmental concern due to their toxic and carcinogenic properties. Development of less costly and less technically challenging remediation methods are needed; therefore, we developed a formulation based on micronized vermiculite that, when air-dried, resulted in a granular product containing the 4-chlorophenol (4-CP)-degrading Gram-positive bacterium Arthrobacter chlorophenolicus A6. This formulation and stabilization method yielded survival rates of about 60% that remained stable in storage for at least 3 months at 4 degrees C. The 4-CP degradation by the formulated and desiccated A. chlorophenolicus A6 cells was compared to that of freshly grown cells in controlled-environment soil microcosms. The stabilized cells degraded 4-CP equally efficient as freshly grown cells in two different set-ups using both hygienized and non-treated soils. The desiccated microbial product was successfully employed in an outdoor pot trial showing its effectiveness under more realistic environmental conditions. No significant phytoremediation effects on 4-CP degradation were observed in the outdoor pot experiment. The 4-CP degradation kinetics from both the microcosms and the outdoor pot trial were used to generate a predictive model of 4-CP biodegradation potentially useful for larger-scale operations, enabling better bioremediation set-ups and saving of resources. This study also opens up the possibility of formulating and stabilizing also other Arthrobacter strains possessing different desirable pollutant-degrading capabilities.

Keywords

Bioremediation; Bioaugmentation; Arthrobacter; Formulation; Microbial stabilization

Published in

Applied Microbiology and Biotechnology
2018, Volume: 102, number: 4, pages: 2031-2040
Publisher: SPRINGER

      SLU Authors

        • Sustainable Development Goals

        Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss

        UKÄ Subject classification

        Microbiology

        Publication identifier

        DOI: https://doi.org/10.1007/s00253-017-8706-6

        Permanent link to this page (URI)

        https://res.slu.se/id/publ/93923