Abstract

Despite recent progress, saccharification of lignocellulosic biomass is still a major cost driver in biorefining. In this study, we present the development of minimal enzyme cocktails for hydrolysis of Norway spruce and sugarcane bagasse, which were pretreated using the so-called BALI(TM) process, which is based on sulfite pulping technology. Minimal enzyme cocktails were composed using several glycoside hydrolases purified from the industrially relevant filamentous fungus Trichoderma reesei and a purified commercial beta-glucosidase from Aspergillus niger. The contribution of in-house expressed lytic polysaccharide monooxygenases (LPMOs) was also tested, since oxidative cleavage of cellulose by such LPM05 is known to be beneficial for conversion efficiency. We show that the optimized cocktails permit efficient saccharification at reasonable enzyme loadings and that the effect of the LPMOs is substrate-dependent. Using a cocktail comprising only four enzymes, glucan conversion for Norway spruce reached > 80% at enzyme loadings of 8 mg/g glucan, whereas almost 100% conversion was achieved at 16 mg/g. (C) 2017 Elsevier B.V. All rights reserved.

Keywords

Lignocellulose; Cellulase; LPMO; Trichoderma reesei; Saccharification

Published in

Journal of Biotechnology
2017, Volume: 246, pages: 16-23
Publisher: ELSEVIER SCIENCE BV

SLU Authors

• Ståhlberg, Jerry

  • The Department of Chemistry and Biotechnology, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Bioenergy


Publication identifier

DOI: https://doi.org/10.1016/j.jbiotec.2017.02.009

Permanent link to this page (URI)

https://res.slu.se/id/publ/94079