Grafting with Arabidopsis thaliana
Melnyk, Charles W.
Generating chimeric organisms is an invaluable way to study cell-to-cell movement and non-cell-autonomous actions of molecules. Plant grafting is an ancient method of generating chimeric organisms and recently has been used to study the movement of hormones, proteins, and RNAs. Here, I describe a simple and efficient way to graft Arabidopsis thaliana at the seedling stage to generate plants with roots and shoots of different genotypes. Using this protocol, success rates of over 80 % with up to 80 grafts assembled per hour can be achieved.
Arabidopsis thaliana; Micro-grafting; Chimeric plants; Mobile molecules; Graft-transmissible signal
Methods in Molecular Biology
2017, Volume: 1497, pages: 9-18
Book title: Plant Hormones: Methods and Protocols
ISBN: 978-1-4939-6469-7, eISBN: 978-1-4939-6467-3
Publisher: Humana Press
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