Abstract

Generating chimeric organisms is an invaluable way to study cell-to-cell movement and non-cell-autonomous actions of molecules. Plant grafting is an ancient method of generating chimeric organisms and recently has been used to study the movement of hormones, proteins, and RNAs. Here, I describe a simple and efficient way to graft Arabidopsis thaliana at the seedling stage to generate plants with roots and shoots of different genotypes. Using this protocol, success rates of over 80 % with up to 80 grafts assembled per hour can be achieved.

Keywords

Arabidopsis thaliana; Micro-grafting; Chimeric plants; Mobile molecules; Graft-transmissible signal

Published in

Methods in Molecular Biology
2017, Volume: 1497, pages: 9-18
Title: Plant Hormones: Methods and Protocols
ISBN: 978-1-4939-6469-7, eISBN: 978-1-4939-6467-3
Publisher: Humana Press

UKÄ Subject classification

Developmental Biology


Publication identifier

DOI: https://doi.org/10.1007/978-1-4939-6469-7_2

Permanent link to this page (URI)

https://res.slu.se/id/publ/94466