Unique genomic configuration revealed by microsatellite DNA in polyploid dogroses, Rosa sect. Caninae

Abstract

An allopolyploid complex with high genomic integrity has been studied. Dogroses transmit only seven chromosomes (from seven bivalents) through the pollen, whereas 21, 28 or 35 chromosomes (from seven bivalents and 14, 21 or 28 univalents) come from the egg cells. Seedlings derived from two interspecific crosses were analysed with flow cytometry and molecular markers to determine ploidy level, mode of reproduction and genomic constitution. Evidence was obtained for the formation of unreduced male and female gametes, which can take part in fertilization (producing seedlings with higher ploidy than the parental plants) or in apomictic reproduction. Random amplified polymorphic DNA (RAPD) and microsatellite analyses indicated that three seedlings (5%) were derived through apomixis, whereas the other 49 were hybrids. Bivalent formation appears to involve chromosomes that consistently share the same microsatellite alleles. Allele-sharing between the maternally transmitted and highly conserved univalent-forming chromosomes reflected the taxonomic distance between different genotypes. The frequently recombining bivalent-forming chromosomes were taxonomically less informative

Published in

Journal of Evolutionary Biology
2006, volume: 19, number: 2, pages: 635-648
Publisher: BLACKWELL PUBLISHING

SLU Authors

• Nybom, Hilde

  • Department of Crop Science, Swedish University of Agricultural Sciences
    

• Werlemark, Gun

  • Department of Crop Science, Swedish University of Agricultural Sciences
    

UKÄ Subject classification

Horticulture
Food Science

Publication identifier

• DOI: https://doi.org/10.1111/j.1420-9101.2005.01010.x

Permanent link to this page (URI)

https://res.slu.se/id/publ/9471