Research article - Peer-reviewed, 2018
ILF2 and ILF3 are autoantigens in canine systemic autoimmune disease
Bremer, Hanna D.; Landegren, Nils; Sjoberg, Ronald; Hallgren, Asa; Renneker, Stefanie; Lattwein, Erik; Leonard, Dag; Eloranta, Maija-Leena; Ronnblom, Lars; Nordmark, Gunnel; Nilsson, Peter; Andersson, Goran; Lilliehook, Inger; Lindblad-Toh, Kerstin; Kampe, Olle; Hansson-Hamlin, HeleneAbstract
Dogs can spontaneously develop complex systemic autoimmune disorders, with similarities to human autoimmune disease. Autoantibodies directed at self-antigens are a key feature of these autoimmune diseases. Here we report the identification of interleukin enhancer-binding factors 2 and 3 (ILF2 and ILF3) as autoantigens in canine immune-mediated rheumatic disease. The ILF2 autoantibodies were discovered in a small, selected canine cohort through the use of human protein arrays; a method not previously described in dogs. Subsequently, ILF3 autoantibodies were also identified in the same cohort. The results were validated with an independent method in a larger cohort of dogs. ILF2 and ILF3 autoantibodies were found exclusively, and at a high frequency, in dogs that showed a speckled pattern of antinuclear antibodies on immunofluorescence. ILF2 and ILF3 autoantibodies were also found at low frequency in human patients with SLE and Sjogren's syndrome. These autoantibodies have the potential to be used as diagnostic biomarkers for canine, and possibly also human, autoimmune disease.Keywords
Lupus-erythematosus; Antinuclear antibodies; T-cells; autoantibodies; dogs; protein; expressionPublished in
Scientific Reports2018, volume: 8, article number: 4852
Publisher: NATURE PUBLISHING GROUP
Authors' information
Bremer, Hanna
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Swedish University of Agricultural Sciences, Department of Animal Breeding and Genetics
Swedish University of Agricultural Sciences, Department of Clinical Sciences
Hansson-Hamlin, Helene (Hamlin, Helene)
Swedish University of Agricultural Sciences, Department of Clinical Sciences
UKÄ Subject classification
Rheumatology and Autoimmunity
Publication Identifiers
DOI: https://doi.org/10.1038/s41598-018-23034-w
URI (permanent link to this page)
https://res.slu.se/id/publ/94735