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Research article2018Peer reviewedOpen access

Molecular markers associated with aluminium tolerance in Sorghum bicolor

Too, Emily Jepkosgei; Onkware, Augustino Osoro; Were, Beatrice Ang'iyo; Gudu, Samuel; Carlsson, Anders; Geleta, Mulatu

Abstract

Background: Sorghum (Sorghum bicolor, L. Moench) production in many agro-ecologies is constrained by a variety of stresses, including high levels of aluminium (Al) commonly found in acid soils. Therefore, for such soils, growing Al tolerant cultivars is imperative for high productivity.Methods: In this study, molecular markers associated with Al tolerance were identified using a mapping population developed by crossing two contrasting genotypes for this trait.Results: Four SSR (Xtxp34, Sb5_236, Sb6_34, and Sb6_342), one STS (CTG29_3b) and three ISSR (811_1400, 835_200 and 884_200) markers produced alleles that showed significant association with Al tolerance. CTG29_3b, 811_1400, Xtxp34 and Sb5_236 are located on chromosome 3 with the first two markers located close to Alt(SB), a locus that underlie theAl tolerance gene (SbMATE) implying that their association with Al tolerance is due to their linkage to this gene. Although CTG29_3b and 811_1400 are located closer to AltSB, Xtxp34 and Sb5_236 explained higher phenotypic variance of Al tolerance indices. Markers 835_200, 884_200, Sb6_34 and Sb6_342 are located on different chromosomes, which implies the presence of several genes involved in Al tolerance in addition to SbMATE in sorghum.Conclusion: These molecular markers have a high potential for use in breeding for Al tolerance in sorghum.

Keywords

Aluminium tolerance; Mapping population; Molecular markers; Net root length in aluminium; Sorghum bicolor

Published in

Hereditas
2018, Volume: 155, article number: 20
Publisher: BIOMED CENTRAL LTD

        SLU Authors

        • Sustainable Development Goals

        SDG2 End hunger, achieve food security and improved nutrition and promote sustainable agriculture

        UKÄ Subject classification

        Genetics and Breeding

        Publication identifier

        DOI: https://doi.org/10.1186/s41065-018-0059-3

        Permanent link to this page (URI)

        https://res.slu.se/id/publ/94950